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Development of an Episomal Plasmid (Minicircle) Plant Engineering System
The Problem: Traditional plastid genome (plastome) engineering is performed using homologous recombination to integrate transgenes into the endogenous plastome of plants. For species with the most efficient tissue culture systems, the complete replacement of the native plastomes with engineered plastomes (homoplasmy) is laborious and lengthy. Therefore,... (read more)