High Throughput Protoplast Isolation and Transformation of Soybean Cells from a Novel Leaf-based Cell Culture-derived System
Published:7/10/2019Description:
The Problem:
To achieve economic feasibility for desirable production, it is necessary to develop systems for rapid screening of gene silencing and genome editing in the plants. A low cost and efficient cell-suspension transformation system that can provide data at an early stage may have potential to eliminate low performing candidate genes/targets prior to the creation of transgenic plants is needed.
The Solution:
Researchers at the University of Tennessee have developed a novel method of isolating and transforming soybean protoplasts using a unique leaf-to-cell suspension culture system, which eliminates several bottlenecks for gene- and promoter discovery.
The method is an inexpensive and efficient way to isolate protoplasts from soybean leaves providing a facile method for high throughput screening optimized for automation. The method of the current invention can be designed for a robotic high throughput transformation and screening of a plant with a plurality of genes of interest and screening the plants to identify and obtain plants having desirable characteristics.
Benefits:
The novel method is more efficient and cost effect than presently used transformation protocols
The method can be designed for high throughput transformation of plants to select for desirable traits.