10 Results for:
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Chemical Roller
“No-Drift” Chemical Applicator The Problem: Unintended herbicide application can devastate home lawns and gardens, as well as surrounding properties. This often occurs due to spray drift when treating for weeds. Products designed to eliminate weeds, but preserve turf grasses, often harm nearby trees, shrubs, and other bedding plants.... (read more) -
Knockdown of miR-21 Expression Sensitizes Cancer Cell to Apoptosis Induced by Interferon
The micro RNA, miR-21, is overexpressed in many human cancers, and accumulating evidence indicates that miR-21 has oncogenic activity. Here we report that the cytokine IFNa/b rapidly induced the miR-21 transcript in several human and mouse cell lines. Pathways known to be involved in regulating cell death were identified as downstream targets for miR-21,... (read more) -
Establishing a murine cell line with high human IFNAR1 expression to characterize type I interferon ligand binding
A mouse cell line was established by transfection that stably expresses the human IFNAR1 chain with various portions of the intracellular domain deleted. The cell line can be used in human interferon bioassays such as ligand binding and antiviral assays. (read more) -
Hybridomas producing monoclonal antibodies to phosphorylase kinase subunits alpha, beta, and gamma
These are mouse hybridoma cell lines that produce IgG monoclonal antibodies to phosphorylase kinase subunits alpha, beta, and gamma. The hybridomas are identified as 157.2, 101.2, and 88.1 respectively. (read more) -
Perceived Activity and Weakness Score (PAWS) as an Indicator of Muscle Weakness
The Problem:Chronic Obstructive Pulmonary Disease (COPD) and Heart Failure (HF) are two leading causes of Healthcare cost in the United States. COPD and HF patients suffer high readmission rates within 30 days post-discharge, increasing overall health-care cost and reducing the quality of healthcare. The Solution: Researchers at the University of Tennessee... (read more) -
My Interactive COPD Mobile Website Application (MI-COPD)
The Problem:Asthma and COPD patients have individual characteristics that make prescribing an inhaler a challenging issue, with each patient having a different set of factors that contribute to which inhaler would be the best fit for them. Sometimes, it takes doctors multiple attempts to find the inhaler that best fits each patient. The Solution: Researchers... (read more) -
Monoclonal anti-DNA antibodies
The invention is related to hybridoma cell lines producing monoclonal antibodies to DNA, which were generated from autoimmune (NZB x NZW) F1 mice. The specificities of the monoclonal antibodies range from low avidity, ssDNA-binding antibodies to high avidity, ssDNA and dsDNA binding antibodies. (read more) -
Use of Recombinant VSV to Study Virus Entry, Glycoprotein Assembly, Immune Responses to Vaccines, and to Identify Cellular Receptors and Virus Entry Inhibitors
A system to recover recombinant viruses from plasmids, and viruses that lack the VSV envelope glycoprotein (delta-G VSV) that also contain reporter genes (GFP, Ds-Red and Luciferase) has been developed. This technology is used to study the entry of heterologous viruses via incorporation of other viral G proteins into replicating (recovered from plasmids)... (read more) -
Autoantibody to Heterogeneous Nuclear Ribonucleoprotein (hnRNP) P2
We generated spontaneous splenic B cell hybridomas and used microscopy to screen for clones reactive with apoptotic Jurkat cells. From hybridomas secreting IgG antibodies reactive with apoptotic cells, we selected one. The antibody, LHC7.15, bound to an antigen that is differentially distributed between the nucleus and the cytoplasm in live and apoptotic... (read more) -
Recombinant Antibody Fusion Proteins and Methods for Detection of Apoptotic Cells
Apoptotic cells contain nuclear autoantigens that may initiate systemic autoimmune response. To explore the mechanism of antibody binding to apoptotic cells, 3H9, a murine autoantibody with dual specificity for phospholipids and DNA, was used. Heavy chain (H) mutants of 3H9 were constructed, expressed as single chain Fv (scFv) in E. coli, and assessed... (read more)