2 Results for:
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Autoantibody to Heterogeneous Nuclear Ribonucleoprotein (hnRNP) P2
We generated spontaneous splenic B cell hybridomas and used microscopy to screen for clones reactive with apoptotic Jurkat cells. From hybridomas secreting IgG antibodies reactive with apoptotic cells, we selected one. The antibody, LHC7.15, bound to an antigen that is differentially distributed between the nucleus and the cytoplasm in live and apoptotic... (read more) -
Recombinant Antibody Fusion Proteins and Methods for Detection of Apoptotic Cells
Apoptotic cells contain nuclear autoantigens that may initiate systemic autoimmune response. To explore the mechanism of antibody binding to apoptotic cells, 3H9, a murine autoantibody with dual specificity for phospholipids and DNA, was used. Heavy chain (H) mutants of 3H9 were constructed, expressed as single chain Fv (scFv) in E. coli, and assessed... (read more)